Abstract
Clearly visualize individual neural structures is a basic principal goal for studying behaviors and underlying neural circuits of living creature in neuroscience. In Drosophila, there have been developed many powerful genetic tool for neural manipulation. Gal4-upstream activating sequence (UAS) system is the most widely used, but Gal4-driver expression patterns are often too widespread and overlap that rarely restrictive enough to map neural circuits. In this study, we use the multicolor fluorescence labeling technique called “Flybow” and “Brainbow” to create a stochastic expression of distinctive fluorescence proteins under Gal4/UAS binary system control. We tested different heat shock conditions to fulfill the most suitable circumstance for distinguish target neurons in the sophisticated Drosophila brain by expressing unequal XFPs, and compare these two genetic tools about their advantages and disadvantages. Our final goal is to achieve the faster neural circuitry map of brainwide connections in Drosophila.