Abstract
Environmental hormones are one of the most often found contaminants produced from commodities. Estrodiol is an endocrine disruptor which has been suspected to cause disruption in the endocrine system. The development of the estradiol-based molecularly imprinted polymer is very important. Molecularly imprinted technique has recently received much attention in chromatography and biosensors. However, most studies focus on the applications and the recognition ability of MIP, not to discuss the relationship between the characteristics and the binding ability. The purpose of this study was to develop and characterize estradiol-imprinted polymer. Estradiol-imprinted polymer was prepared in bulk polymerization by non-covalent binding. Estradiol and methacrylic acid were used as template and functional monomer, respectively and the optimal solvent used to synthesize by chloroform. The maximum adsorption amount for estradiol is 0.7998 mg/g. However, the MIP synthesized in chloroform with high ability has low surface area. The result of characteristics can explain this phenomenon and prove that the binding ability is positively correlation to active site, not surface area.