Abstract
DNA microarray experiment is a high throughout technology to assay gene expression level that is proportional to the intensities. Accurate image analysis is important to explore differential expressed genes. Different image analysis will influent statistical conclusion. In our study, we segment image by two-dimensional K-means with removal of unreliable pixels to obtain more accurate estimates. We compare our results with those obtained by mixture model. Our method results in higher correlation and consistent variance across replicates.