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The Effects of CAP2 Overexpression on Mitochondrial Dynamics and Cell Migration in Cancer Cells
Thesis

The Effects of CAP2 Overexpression on Mitochondrial Dynamics and Cell Migration in Cancer Cells

Shih, Yu-Ying
Masters, 國立清華大學, 生物科技研究所
2012

Abstract

粒線體動態平衡 人類線苷酸環化酶關聯蛋白質二號 細胞遷移 表皮子宮頸癌細胞 胃癌細胞 Mitochondrial dynamics CAP2 cell migration HeLa cancer cells SC-M1 cancer cells
Mitochondrial dynamics is important for mitochondrial functions and cell migration in cancer cells. Previous studies showed that elongated mitochondria elevate mitochondrial membrane potential, enhance mitochondrial respiration, and decrease cell migration; fragmented mitochondria decrease mitochondrial membrane potential, reduce mitochondrial respiration, and promote cell migration. CAP2 sequesters G-actin and disrupts actin filaments. Moreover, CAP2 is up-regulated in hepatocellular carcinoma, and the knockdown of CAP2 suppresses the cell migration. However, there is no study discussing the correlation of CAP2 with both mitochondrial dynamics and cell migration in cancer cells. In this thesis, I found that CAP2 overexpression affected mitochondrial dynamics, and caused mitochondrial morphology and functions were changes in both HeLa and SC-M1 cancer cells. In addition, CAP2 overexpression disturbed actin filaments and decreased the activity of cell migration.

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