Abstract
To identify cold-inducible genes in mungbean, we prepared a mungbean cDNA set containing independent cDNAs combined with 391 cDNAs isolated from 3 day old seedlings and 621 cDNA from subtraction for cold- and heat-treatment. This study focused on some of the candidate genes obtained from our preliminary microarray data. We aimed to study the mungbean genes involved in the ubiqitin/26S proteosome pathway from our cold-subtracted library, including the ubiquitin, ubiquitin conjugating enzyme, RING type ubiquitin ligase, 20S core protease, and 19S regulatory particle. We confirmed those genes using Northern blot analysis and finally identified the cold-induced ubiquitin conjugation genes. These results suggest that the ubiquitin conjugation genes have roles during cold stress through the turnover of protein(s) via the ubiquitin/26S proteasome pathway, but not heat.