Abstract
Type 2 diabetes mellitus (T2DM) is a metabolic disorder characterized by insulin resistance. Reactive oxygen species (ROS) can suppress the insulin response and contribute to insulin resistance in adipocyte. Glucose transporter 10 (GLUT10) encoded by the SLC2A10 gene is a member of the class III facilitative glucose transporter family. In our lab, we found GLUT10 was localized in the mitochondria after insulin-stimulated in adipocyte. In the unstimulated adipocyte, GLUT10 was localized in the Golgi apparatus. GLUT10 is confirmed as a DHA transporter in mitochondria in aortic smooth muscle cell (A10) and insulin-stimulated adipocytes (3T3-L1). DHA is the oxidized form of ascorbic acid. Ascorbic acid (also called vitamin C) can against oxidative stress and induce to decreasing of ROS level in cell. When the GLUT10 targeted to mitochondria by insulin-stimulated, it can reduce ROS level. The cells fail to respond to insulin in insulin resistance. Previous studies have shown that elevated ROS level in mitochondria can inhibit insulin signal pathway and glucose uptake. Therefore, we will understand the role of GLUT10 in insulin signal pathway, glucose uptake and ROS scavenger in adipocyte.