Abstract
Tricarboxylic acid cycle (TCA cycle) is an essential metabolism pathway and plays an important role in homeostasis and lifespan regulation. We uncovered a mutant fly NIG 9438R-1 which displays extended lifespan and up-regulation of CG32026. CG32026 is predicted as isocitrate dehydrogenase-like gene, which may metabolize isocitrate to alpha-ketoglutarate in TCA cycle. Feeding of alpha-ketoglutarate has been reported to extend lifespan via TOR (target of rapamycin) pathway in C. elegans. It is not reported whether CG32026 plays a role in the regulation of lifespan and stress tolerance in Drosophila. Here, I demonstrated that knockdown of CG32026 (isocitrate dehydrogenase-like gene) might reduce lifespan and modulates starvation and oxidative tolerance in female flies. In addition, the expression of CG32026 negatively modulates the levels of the phosphorylated S6 kinase (p-S6K) in Drosophila, suggesting that the isocitrate dehydrogenase-mediated lifespan and stress tolerance may be involved in TOR pathway. Together, the data indicate that CG62026 isocitrate dehydrogenase-like gene regulates lifespan and stress tolerance in Drosophila.