Abstract
Abstract Xanthomonas campestris pv. campestis is a Gram-negative bacterium and one of the most important plant pathogens. It attacks cruciferous plants and causes worldwide agricultural loss. We annotate 575 contigs of X. campestris pv. campestis, which were sequenced by Genome Research Center at NYMU. We got 6327 and 3811 ORFs (open reading frame) predicted by Glimmer 2.0 and ORPHEUS. Then we performed BLAST search against Genbank nr (non-redundant) protein database and there were 5854 ORFs having similarity with protein sequences in the database ( sequence identity > 30% ). We did the sequence search with COG (clusters of orthologous groups) database for protein function assignment, and we got 2819 hits in our ORFs (E-value cut-off = e-20). The pathogenesis mechanism is one of the most important issues for plant pathogen study. 487 candidate genes are found responsible for pathogenesis after comparing our sequences with the annotations of the other two plant pathogens, Xylella fastidiosa and Ralstonia solanacearum. This result could apply to study the pathogenesis mechanisms of different plant pathogens and those genes could be good targets for structure determination. We collected all annotation results to create a X. campestris pv. campestis gene annotation database ( http://xcc.life.nthu.edu.tw ). This database can help the research related to X. campestris pv. campestis and our final goal is to select the valuable target proteins for structure genomics .