Abstract
Abstract One of the hallmarks of memory formation is that memories initially are stored in a short-lived labile form, but with time can be transferred into longer-lasting forms that are resistant to experimental manipulation. In both vertebrate and invertebrate animals, anesthetic agents cause retrograde amnesia for recently experienced. Genetic studies in Drosophila indicate that anesthesia-resistant memory (ARM) is a genetically distinct form of consolidated memory that exists in parallel with CREB-dependant long-term memory (LTM). Unlike LTM, ARM is disrupted in radish mutant flies but is not affected by manipulation of the CREB gene. ARM also is resistant to inhibitors of protein synthesis and is formed more rapidly following the learning event than is LTM. ARM nevertheless is a long-lasting memory that can persist for more than 24 hours. To date nothing is known in any species about the molecular, genetic, or cell biological pathways underlying ARM. In unpublished data reveals that rshC133 enhancer trap line is another allele of rsh. In this study, I observe that rshC133 reveals expression in a novel anatomical pathway. Notably, radishC133 reporter is not expressed in mushroom bodies, the primary anatomical focus of olfactory memory research, suggesting that ARM relies on a distinct anatomical structure.