Abstract
Abstract--------------------------------------------------------------------------------------------I Acknowledgment ------------------------------------------------------------------------------III List of Figures------------------------------------------------------------------------------------V List of Tables------------------------------------------------------------------------------------IV Introduction---------------------------------------------------------------------------------------1 Materials and methods---------------------------------------------------------------------------8 Results--------------------------------------------------------------------------------------------13 I. Microarray data analysis-------------------------------------------------------------13 II. Validation of microarray data by in vivo experiments--------------------------20 III. Synergistic effect on lifespan between the EP longevity lines and other long-lived mutants---------------------------------------------------------------------22 Discussion---------------------------------------------------------------------------------------26 I. Genomic analysis of three EP longevity lines------------------------------------26 II. Genetic analysis of three EP longevity lines-------------------------------------31 Figures and Tables------------------------------------------------------------------------------34 Reference----------------------------------------------------------------------------------------62 Appendix: Plasmid rescue and inverse PCR------------------------------------------------68