Abstract
English abstractWhile cytotoxic effects of individual gallium arsenide were well documented, the direct investigation of cytotoxic effects of dissolved gallium arsenide compound is largely unexplored.Here I showed that the cytotoxic and genotoxic effect of the long-term gallium arsenite treatment of human fibroblast cells. I also compare the influence on human fibroblasts after sodium arsenite treatment to gallium arsenide treatment.This study also proves that cells increase cellular ROS levels when human fibroblast cells were treated with these chemicals for 4 h and 12 h. In my laboratory, it had been examined that short-term exposure (4 h) of human fibroblast cells to gallium arsenide exhibit genotoxic micronuclei (MN) formation, and cytotoxic effect. Further more, I also demonstrated that long-term exposure (12 h) of human fibroblast cells to gallium arsenide exhibit genotoxic micronuclei (MN) formation, and cytotoxic effect.In addition, the p53, HO-1 and p21 protein expression was examined after 0-80 μM sodium arsenite or gallium arsenide treatment. Generally, p53 expression raises at 0-40 μM NaAsO2 and GaAs. When cells exposed to arsenic compound, largely induce HO-1 expression.After sodium arsenite or gallium arsenide treatment for 4 h, the number of cells at the cell cycle arrest at G2/M phase increases with the treatment dose. After 40 μM sodium arsenite treatment for 12 h, partial cells of a population precede program cell death. The gallium arsenide treatment groups have this phenomenon until the gallium arsenide concentration increase to 80 μM。