Abstract
Abstract:WTK1 and TK6 cell lines were derived with both the same mutagenic treatment and mutation selection from different colony-forming subclones of the spleen nonclonal lymphoblastoid isolate--WI-L2. We found that there was a lower sub-G1 cell ratio in WTK1 cell than TK6 cell after incubation in fresh medium for 24 hours with the previous identical concentration 4 hours treatment of sodium arsenite (NaAsO2) or antimony chloride (SbCl3) while the same intake and accumulation of arsenite was performed.Basing on the difference in cytotoxicity response of WTK1 and TK6 cells after sodium arsenite or antimony chloride treatment, the alternations of NO (nitric oxide), GSH (glutathione), GPx (glutathione peroxidase), and catalase induced by the treatment were examined. WTK1 cells performed a stronger resistance toward sodium arsenite and antimony chloride to TK6 cells in views of sub-G1 cell ratio raise and cell viability decrease. After the 4 hours treatment of arsenite or antimony, there were intracellular RNS/ROS (reactive nitrogen species/ reactive oxygen species) increases in both WTK1 and TK6 cells. With the treatment concentrations of sodium arsenite or antimony chloride raising, the intercellular NO levels were increased more in WTK1 cells, the intracellular GSH amounts were decreased only in TK6 cells, and the GPx specific activities of both cell lines were increased while no obvious change was seen in catalase. The catalase specific activity change of cells incubated in fresh medium after the 4 hours treatment of sodium arsenite or antimony chloride was found to be responsively decreased with different drug concentrations, and larger decrease was seen in TK6 cells in the same treatment. However, after the co-treatments of NAME (N□-nitro-L-arginine methyl ester), SNP (sodium nitroprusside), BSO (L-buthionine-[S, R]-sulfoximine), MS (mercaptosuccinic acid), and AT (aminotriazole) with sodium arsenite or antimony chloride respectively, it was found that only the co-treatments of NAME, SNP and BSO had effects on cell viability decrease.To sum up, in this thesis, it was suggested that NO and GSH might be involved in the difference of WTK1 and TK6 cell lines toward sodium aresnite and antimony chloride treatments. Intracellular GPx and catalase were changed by the treatments, but their changes might not be involved in the difference.中英摘要…………………………… 1簡 介…………………………… 5實驗方法……………………………13結 果……………………………16討 論……………………………22參考資料……………………………27圖 表……………………………41附 錄……………………………59