Abstract
This study presents an imitation of oviduct microfluidic dielectrophoresis chip system. In order to reduce the impact and destruction of cells, this study adopts dielectrophoresis force to manipulate the cell. Imitation of IVF (In Vitro Fertilization), the oocyte through the DEP force positioned in the microfluidic channel, and then we trap much more sperms around the oocyte by dielectrophoresis force to enhance the probability of natural fertilization. Therefore able to oocyte positioning in the microchannel and sperm with the fluid also go the same direction, so that the much more sperms around the oocyte can enhance the probability of natural fertilization. Dielectrophoresis force primarily through non-uniform electric field induces the electric field distribution. According to the differences between of particle and solutions’ dielectric coefficient and conductivity induce difference dipole moment in the electric field. Different polarization characteristics of ability of particles and solution to generate positive or negative dielectrophoresis effect, the particles will be affected by the DEP force to move to the region with high or low electric field density to achieve the goal of oocyte positioning. The positive dielectrophoresis response of oocyte was exhibited with the frequency at 1 MHz, the oocyte will be affected by the DEP force to move to the region with high electric field density. To understand the position with high and low electric field distribution in microchannel, the numerical commercial software, CFDRC-ACE+, was used to know where the high electric field is. This study is divided into two parts, the first to complete the insulator structure of microchannel dielectrophoresis chip system fabrication and setup. Mainly we manipulate cell in the microchannel, which contains the chip design and fabrication, dielectrophoresis electric field simulations, dielectrophoresis cell manipulation results. The second part improves the dielectrophoresis chip, which enhance the probability of oocyte position and sperm collision oocyte.