Abstract
This study bases on the technique, “Electrowetting on Dielectric (EWOD),” which is actuated by addressable coplanar electrodes for micro droplets manipulation. Multiple bio-reagents such as DNA, magnetic beads and buffer have manipulated synchronous with different concentration. All of the droplets are driven with water oil core-shell type, which are surrounded by silicon oil. All the droplets are generated homogeneously with ultra microvolume(0.3μL). A coplanar EWOD chip with several reservoirs and electrodes, is fabricated by indium tin oxide (ITO) glass, which can drive droplets for creating, transporting, merge and splitting. All the fluidics movement is confined between two plates, a bottom plate for the coplanar chip, and another hydrophobic plate with SU8 structure for the top. Detections and capturing of images are easy and convenient through transparent ITO glasses, which are well characteristic of optic. Through the construction of this addressable coplanar-electrode EWOD chip, the study demonstrates a flexible platform for Digital Micro Fluidic (DMF) Systems to manipulate micro droplets. To perform a bio-application on the EWOD chip, we transfer a DNA ligation process on this device, which is a common procedure for biomedical analysis. Droplets with magnetic beads are also manipulated on the chip. Moreover, a probe formation process of SNP detection is also completed which confirmed by QDots labeling. The result shows that probes synthesized by EWOD chip are better than by hand. Thus, in this paper, we wish the device could be integrated with other systems to achieve the goal of Micro Total Analysis System (μTAS) and Lab on a Chip.