Abstract
Abstract In this study, we first generated a panel of mouse monoclonal antibodies against extracellular domain of human CD63, a novel member of a recently identified protein family, tetraspanins. One of these monoclonal antibodies is found to specifically bind to mouse CD63, suggesting a self-responsive immune response. We also tried to investigate the function of CD63 in two ways: (1) an inhibition of CD63 expression by transfecting inverted CD63 extracellular domain expression vector into mouse macrophage cell line, Raw264.7; (2) Yeast Two Hybrid System to screen the possible protein associations with CD63 extracellular domain from spleen cDNA library. The rationale design is based on our assumptions that CD63 may be related to the basic cell physiology of macrophages-say phagocytosis, antigen presenting, and cytokines secretion. On the other hand, we speculate that extracellular domain of CD63 can play some important role on the plasma membrane, and these functions may not easily revealed by the approaches that have been previously used to understand CD63 function (effects of CD63 antibody binding, and co-immunoprecipitation). Actually, through yeast two hybrid system, the identified proteins interacting with CD63 are totally different from those reported previously: some lysosome (such asα-mannosidase ) or phagocytosis (such as macrophage receptor with collagenous structure and C1q beta chain )-related proteins were found. We propose that CD63 extracellular domain may play an important role in the process of phagocytosis.中文摘要Chinese Abstract……………………………….……1英文摘要 English Abstract…………………………………….2簡介 Introduction………………………………………………3目的 Specific Aims……………………..………………….…19材料與方法 Materials and Methods…………..……………..19結果與討論 Results and Discussions…………….…………..36參考資料 References……………………..…………………..57附錄一 Appendix I附錄二 Appendix II附錄三 Appendix III附錄四 Appendix IV