Abstract
In this research, we develop a method of using solid-phase microextraction (SPME) coupled to high performance liquid chromatography (HPLC) to detect five diphenyl-ether type herbicides. They are aclonifen, bifenox, fluoroglycofen-ethyl, oxyfluorfen and lactofen.Solid-phase microextraction is a new technique developed by Pawliszyn in 1989. The unique feature of this technique is that it combines sampling, extraction, concentration and sample injection in one step. It’s faster and simpler than other traditional pretreatment technique. It is also more friendly to our environment because it uses no organic solvents. In this thesis, we develop a method of using this with HPLC to detect the above five compounds. We investigate some variations that affect the efficiency of extraction including extraction fiber, extraction time, soaking time, desorption mode, component of desorption liquid, extraction temperature, stirring rate, carryover, sample pH value, organic solvent and salt addition. We also check it’s linearity correlation coefficient, relative standard deviation and detection limit.We choose PDMS/DVB and CW/TPR these two fibers to detect water samples in our environment. The recovery of these two fibers are all above 80 % ( 81~104 % for PDMS/DVB and 83~100 % for CW/TPR fiber ), this shows that the interference from this water sample matrix is not too severe. The detection limit for PDMS/DVB is 0.33 ~ 1.74 ng/mL and CW/TPR 0.22 ~ 1.94 ng/mL. Finally, this thesis offers a way of detecting diphenyl-ethercompounds in water samples by SPME/HPLC.