Abstract
Abstract All functions in biological system are almost accomplished by transduction of electromagnetic signal; the binding energy among these biological molecules is larger than that of microwaves and extremely low frequency(ELF) electromagnetic(EM) fields. The energy of EM waves is incapable of breaking the bindings, but affect the electrons within the molecules for their polarization and drift, etc. So the cell properties may be influenced by the EM waves. In this dissertation, we utilized the free radicals that were generated by from Co-60 or hydrogen peroxide to attack the DNA of the V79-4 cell. The DNA repair-gene was triggered to repair the free radical damaged DNA. Meanwhile, The EM waves irradiated the target cell. The 2-[4,5– Dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay presented the surviving fraction of the cell. The MTT powder changed the color from yellow to purple by an interaction between the reagent and the electrons in the electron-transport chain on the mitochondria. The optical density (OD) value retrieved from MTT assay deduced the surviving fraction of the cell. The results indicated that the ELF EM wave had no influences on the surviving fraction of the cells that were damaged by Co-60 or hydrogen peroxide. However, when the microwaves irradiated on the DNA-damaged cells, the statistical test clarified that the surviving fraction of the cell decreased significantly. In order to confirm the DNA-damaged cells were sensitive to EM waves, the microwaves and ELF EM waves were irradiating the cells that the DNA was not damaged. After obtaining the surviving fraction of the undamaged cell the experiments indicated that surviving fraction had no significant variation. In addition, we measure the DNA damages by single cell gel electrophoresis method. Nonetheless, the slight dose irradiator of Co-60 or hydrogen peroxide performed no effect in the strategy. The experimental conditions, such as the electrophoresis time and running buffers, could be further optimized in the future research.