Abstract
The ability of wound healing for organisms is important. The downstream of JNK signaling pathway, puckered, was activated in wound healing process and expressed around the wound. We make a transgenic fly pucF43-pGP including the upstream 1.6 kb non-coding DNA sequences of puc and expressing pucF43-GFP in cytoplasm. This DNA fragment showed many AP-1 transcriptional factor binding sites by TESS. We inserted the upstream 0.7 kb (pucF3) and 0.7-1.6 kb (pucF4) non-coding DNA sequences of puc into pH-Stinger vector which expressing nuclear GFP to generate pucF3-pHS and pucF4-pHS transgenic flies. These two transgenic flies had no difference in GFP expressing pattern, GFP intensity and the speed of wound healing with pucF43-pGP fly significantly. We took pucF43-pGP transgenic fly to screen genes related to wound healing. hep1 increased the GFP intensity, and slowed down the speed of wound healing. ed mutant didn’t affected GFP expressing pattern or GFP intensity, so that we can not prove that ed related to wound healing.