Abstract
Once the S. aureus biofilms disperse and flow into the blood, the clusters of S. aureus will cause serious disease, even death. Finding effective antifouling surface for catheters is an important issue to reduce possible problems that can be induced by bacterial infection. This study was conducted in two situations, with S. aureus cultivated solution in static mode and in flow mode. S. aureus was cultivated for 21 days to observe the biofilm development on patterned PDMS substrates with flat, aligned, and unaligned surfaces. The purpose of the study is to investigate the development of bacterial attachment and colonization on an engineered topography with a well-defined pattern. We are concerned with the design and characterization of surface microtopographies that effectively control bioadhesion. The results of this experiment showed that topographical surfaces have the ability to decrease S. aureus attachment and colonization. In flowing mode, unaligned patterned surfaces disrupted the colonization and formation of biofilm. The percentage of the area coverage for S. aureus on flat, aligned patterned, and unaligned patterned surfaces are 48.2 %, 20 %, and 10.3 %, respectively. In static mode, both aligned patterned and unaligned patterned surfaces decrease the colonization percentage. However, the effectiveness of unaligned patterned surfaces on the reducing of the S. aureus attachment is about the same as that for the aligned patterned surface.