Abstract
In this study，we investigate the role of low molecular weight chondroitin sulfate type-A (LMW-CSA) on the expression of interleukin-1 beta(IL-1β)-mediated gene expressions of osteoarthritis in cultured human chondrosarcoma (SW1353) cells. Quantitative real-time polymerase chain reaction (Q-PCR) was used to analyze MMP-1、MMP-13 and IL-1β gene expressions after treating SW1353 with IL-1β.Results show that IL-1β-induced MMP-1 and IL-1β gene expressions were not altered in the presence of mixture of 2、4、6、8、10、12、14 units of LMW-CSA . However IL-1β-induced MMP-13 gene expression were reduced when the mixture of 2、4、6、8、10、12、14 unit LMW-CSA was given. We also observe protein level of MMP-13 was reduced after the addition of mixture of 2、4、6、8、10、12、14 units LMW-CSA. Further analysis shows that mixture of 10-14 units of LMW-CSA is more effective in the inhibition of MMP-13 gene expression and protein level. Our results also suggest that the inhibitory effect is not through p38 and JNK1 pathways.