Abstract
Abstract In this study, we investigate the role of LMW-CSA, LMW-CSC or IL-1□-mediated gene expression in cultured human articular chondrocytes. We analyzed IL-1□-mediated gene expression by quantitative real-time polymerase chain reaction (Q-PCR), MMP1, ADAMTS5, COL2A1, AGC1, COX1, and COX2 were induced, but COL1A2 were down-regulated by IL-1□ treatment. We also observed a morphological change after treating with IL-1□. However, this morphological change of IL-1□□exposed cannot be recovered in the present of LMW-CSA or LMW-CSC. As analyzed by Q-PCR the IL-1□-induced MMP1 and ADAMTS5 gene levels can be administration of LMW-CSA, but not LMW-CSC. Althout LMW-CSA reduce IL-1□-mediated or basal level of MMP1 gene expression by Q-PCR, these inhibitory effect are not acted through p-ERK1/2 pathway. We also investigate whether Intact-CSA is able to reduce IL-1□-mediated MMP1 and ADAMTS5 gene expressions. The result showed that Intact-CSA could not inhibit IL-1□-induced MMP1 and ADAMTS5 gene level.