Abstract
We made a variant automated chemostat. The devices have small working volume of ~10 ml and low-cost different from the traditional bioreactor culture. The devices are built based on commercially available electronic components such as Ardunio board, light emitting diodes, and micropumps. The operation of the whole system is controlled with Arduino board automation. Using light emitting diodes measurement absorbance of bacteria in culture environment, observing the number of growth bacteria in real-time. Inject fresh medium and increase drug by micropump. Simultaneously, dynamically adjust the drug concentration according to growth of the bacteria. Allowing bacteria evolve mutant with high antibiotic resistance. The device we call the ’morbidostat’.