Abstract
This paper is to discuss a novel EWOD chip and mouse embryos cultured IVF (in vitro fertilization) as a EWOD chip. EWOD platform placed in temperature-controlled intravital microscopy system and provides temperature and gas concentration required for cell growth, in real time, high resolution microscope observation monitoring the situation of cell growth. They were divided into two groups, static culture group and dynamic culture group. In dynamic culture group, EWOD chip for oocyte and sperm fertilization combined droplets, moving and observation.The Dynamic group step 1 is to draw HTF medium containing 0.08% pluronic and the drop in the dielectric and humid electrodes on the chip and cover oil. Step 2 is to use dielectric wafer wet sperm droplet beads mixed with sperm in the fluid motion. Step 3 the droplet moved on five EWOD electrodes dynamic culture. The period of each move will take 30 minutes, and every move will be finished within 30 seconds by using the operating voltage of 60V square wave frequency of 500 Hz. Experimental results show the dynamic culture and static culture group, embryos fertilized embryos similar probability. The results confirm that EWOD systems can be used on fertilized embryos feasibility of reproductive medicine.