Abstract
This research proposes a photochemical method to dissociate immune complexes, decrease the hurt to protein of traditional acid washing by extremely shot operation time. We used the well-known characteristic of o-NBA molecules that can be photoinduced proton releasing by 365nm irradiation and make pH jump in the solution to dissociate immune complexes. In order to prove our proposal, we set up a flowing system, fixed antibody which has fluorescence and antigen modified with quencher which can quench the fluorescence in it. Because the effective distant of quencher quenches fluorescence is very shot (~10nm), only when antigens binds to antibodies, the distant is shot enough to quench the fluorescence. We judgment if antibody and antigen bind or not by fluorescence was quenched or not. The fluorescent dye we chose was quantum dots because of its fluorescence won’t be influenced by the pH value changes in the environment. Base on our results of experiments this method is workable, and it got similar recovery ratio to the solution pH=3.