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光還原金奈米粒子於DNA生物高分子之螢光能量轉移特性研究
Thesis

光還原金奈米粒子於DNA生物高分子之螢光能量轉移特性研究

饒瑞桓
Masters, 國立清華大學, 光電工程研究所
2015

Abstract

金奈米粒子 去氧核醣核酸 福斯特共振能量轉移 局部表面電漿共振 gold nanoparticles deoxyribonucleic acid, DNA Förster resonance energy transfer, FRET localized surface plasmon resonance, LSPR
Förster resonance energy transfer (FRET) has been widely applied in biomedical sensing studies. The purpose of our study is to explore potential FRET-mediated applications in optoelectronics by combining the fabrication platform of deoxyribonucleic acid (DNA) composite and photoinduced gold nanoparticles (NPs). In this study, we first examine the FRET efficiency in dye-doped DNA system and show that the efficiency could be enhanced by virtue of the structural features of DNA compared to the PMMA counterparts. It is suggested that the structure of DNA can spatially organize the chromophores and reduce the phenomenon of self-quenching due to the aggregation of chromophores. Furthermore, we examine the influence of localized plasmon resonance in the FRET systems by addition of gold NPs synthesized by photoreduction method. We analyze the optical properties of gold NPs with different photoreduction time by absorption spectra and the shape of gold NPs with different photoreduction time by transmission electron microscopy (TEM). Finally, we investigate the FRET systems with different doping levels of gold NPs by photoluminescence (PL) spectra and color analysis in CIE color space. We present that color of the thin film can be changed with different doping levels of gold NPs by FRET. Such effect can be used in the applications of UV sensors and various photonic devices.

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