Abstract
Antrodia camphorata ( A. camphorata ) is well known in Taiwan as a folk medicinal fungus with anticancer and anti-inflammatory effects. However, the molecular mechanism underlying leukemia growth inhibition effects through different A. camphorata treatment is poorly understood. In this study, we constructed an integrated high-throughput platform to standardize the herbal materials by using a combination of herbal identification, UV-VIS spectrophotometry, high performance liquid chromatography (HPLC), and inductively coupled plasma mass spectrometry (ICP-MS). Simultaneously, we used cDNA microarray technology to examine the effects of various A. camphorata mycelia extracts (water and methanol extracts) on the effects of growth inhibition on KG-1 cells (human acute myelogenous leukemia cell line). Based on this science-based platform, we developed a practical quantitative method and established an organic/inorganic chemical fingerprint. By the comparison of KG-1 global gene expression patterns from herbal extracts, several possible biological responses and relevant marker genes were identified by gene ontology (GO)-based clustering analysis. The statistically significantly comparison of GO category from both treatments indicated that water extract could down-regulate carbohydrate catabolism related genes and methanol extract up-regulate MHC class II antigen processing genes, respectively.