Abstract
Abstract Japanese encephalitis virus belonging to flavivirus infects human by mosquitos. The domain III of envelope protein is regarded as to integrate the surface protein receptors from the host cell .The non-structure protein(NS2b3-180a.a) not only modifies protein but also relate the cellular apotosis. This study intends to identify cellular factors interacted with Japanese encephalitis virus (JEV) using phage display technology,and then use ELISA and co-immunoprecipitation to further confirm the function. The result of Biopanning one group of ED3 associated proteins is relation with the mitochrondrial ribosomal protein L34 with 100% identy, the other group contains the GPP motif reported in the collagen. Functional interaction of ED3 protein with cellular factors ED3-17 and ED3-46 was examined using ELISA,co- immunoprecipitation and plaque assay reduction test. Inaddition, the immunofluorescence staining of neuron cells was tested using the rED3-17 and rED3-46 immunized sera .The Biopanning result also indicated that most NS2b3-associated proteins show a motif,which was found in dentatorubral-pallidoluysian atrophy (DRPLA). Apoptosis of the Neuron cells transfected with NS2b3-pcDNA3.1 was observed.The findings supported the induction of apoptosis by NS2b3 protein. Our results will be useful for understanding the molecular pathogenesis of Japanese encephalitis .