Abstract
In the recent years, the effect of trace element on human health attracted much of the attention. Such elements has strong influence in human body biochemical reactions, it participated in the bio-molecular formation with special effects. A clinical medicine studies on the trace elements was the focus of international research, and became a clinical treatment directions.This study was aimed to upgrade the medical research in Taiwan, with in depth study of trace element on human body functions, from the point of views of analytical chemistry, sample collection, pollution control and instrumental identification to make evaluations. In order to develop a new clinical technique for simple, fast, highly reliable analytical procedure, we want to identify and analysis the trace elements in human urine. This study applied Multi-Element, Graphite Furnace Atomic Absorption Spectrometer (SIMMA 6000), to determine directly and simultaneously the compositions of Bismuth, Cadmium, Lead, Selenium and Antimony in Urine. Due to the protein and non-organic salts could interference against direct measurements, and the five elements are only trace in amount in the urine, thus we added very limited amount of Bismuth, lead, cadmium, selenium and antimony into the urine sample, plus appropriate amount of palladium (Pd) modifier, to determine the best possible process and the optimal analyze conditions. During the analytical process, hydrogen was introduced to prevent palladium particle precipitation and forming large particles aggregate, to make palladium particle smaller with even distribution. To improve palladium’s ability for stable analysis, for improved atomized efficiency. This study already applied simple non-ionized water (with 0.5% HNO3), successfully identified the optimal conditions to analyze the trace elements of Bismuth, Cadmium, Lead, Selenium and Antimony in human urine, and the practical application of clinical human urine analysis.In the future, it is possible to apply corrected curve technique and standardized modifier addition to analyze quantitatively the four elements in the human urine, to identify the test limits of Bismuth, Cadmium, Lead, Selenium and Antimony, in order for application in the practical clinical medicine.