Abstract
The direct and simultaneous determination of copper, chromium, aluminum and manganese in urine using multielement graphite furnace atomic absorption spectrometer ( Perkin-Elmer SIMAA6000 ) are described. Pd is the chemical modifier. The matrix interference not only could be removed completely by dilution: 1+1, but also the optimal temperature program. A simple calibration curve method could be used to determined Cu, Cr, Al and Mn in SeronormTM Trace element urine. The analyzed values were within the 10% range of the certified values. From the analysis of SeronormTM Trace element urine and observation of platfrom by SEM, using 5%H2 and 95%Ar gas as the purge gas during drying and the first ashing steps could make Pd particles smaller and more well-distributed so that increase the function of Pd chemical modifier and promote the efficiency of atomization. For measurement of the real human urine, the recovery studies of Cu, Cr, Al and Mn were 100±20%. The relative standard deviations (RSDs) for these four elements were 1.7%, 1.5%, 1.6% and 1.5% and the detection limits were 0.08 μg l-1 for Cu, 0.05 μg l-1 for Cr, 0.06 μg l-1 for Al and 0.06 μg l-1 for Mn.