Abstract
Abstract In this study, optical immune sensor chip can be used in medicine, biochemistry related to the detection, the use of special nano-arrays, allowing the signal enhancement, to achieve rapid screening function. This study includes nano-micron manufacturing technology of gold array structure, chemical synthesis of particles, surface chemical modification techniques and fluorescence signals and Raman signal testing technology. Biological detection chip has been studied for many years, but a small number of samples to achieve can be fast and clear signal that we need research in this area has been subject in recent years, many people in the study of nanostructures for specific enhanced Raman signal, if able to use the above method, can reduce the test material and to increase the signal. The aforementioned nano optical immune sensor chip is a layer of surface modification by metal particles self-assemble monolayer, monolayer and then the above functional groups and the antibody binding functional group, each between antibody and antigen identification features achieve sensing mechanism. In order to increased the signal and less target in our chip, the study design was a three-dimensional rough surface structure level, with different parameters, which can effectively increase the signal detected by the Raman spectroscopy results can be verified, Therefore, through this technology, not only effectively reduce the amount of samples, and help enhance the Raman signals.