Abstract
The ability of self-repaired limited in damaged chondrocytes has been an important issue in medical development. We must create more chondrocytes sources to encounter this problem. The sources of chondrocytes come into two main parts, the first one is differentiated from messenchymal stem cells, the other is culturing chondrocytes in vitro from autologous transplantation. Messenchymal stem cells has becoming chondrocytes research main parts recently due to its high self-renewal and differentiation capabilities. However, if we can find way to culture high production chondrocytes in vitro, it will provide a more directly method to solve the problem. Here we propose an intuitive method to test cells proliferation by giving whole cells a homogeneous extension force and then release it to the original state .We intent to repeat this regular movement cycle to increase cells growth rate. We incubated rabbit chondrocyte primary cells on PDMS(Polydimethylsiloxane) nano wells which can deliver homogeneous controllable stress at regular vibration cycle. Due to PDMS is a hydrophobic material, we used both oxygen plasma and poly-L-lysine treatments ensuring cell adhesion result. Uniform cells were grown inside the wells and underwent different stress and cycling conditions, and it was found that a 3% strain at 0.75Hz continuously stretching of 2 hours for two days was most helpful for bone cells interior activity in plane well. Afterward we add nano-structure on plane well to examined nano-structured irritation on chondrocytes behavior. Results showed structured can enhanced proliferation rate compared to plane well in static state, however when we applied mechanical stimulation on both group it would not have much difference.