Abstract
In recent years, our lab set up a condition for isolated subunits of the crystalline cell surface layer (S-layer) protein from Deinococcus radiodurans IR and purify this protein efficiently. Up to now, we developed a new method for high-yield preparation of S-layer fused with liposome (SLL) and could improve liposome instability. We used simple and convenient method to make SLF (S-layer fraction) fuse with liposome. Isolated subunits of the negatively charged SLF were fused with positively charged liposome not only they fuse together dependent on electric potential，but also they take advantage of lipid membrane fusion effect. We can observe SLF and SLL under confocal laser scanning microscopy and Zeta-Sizer .Furthermore，we had investigated and analyzed the stabilizing effect of SLL towards acid、base or osmotic solutions by Zeta-Sizer. We have demonstrated the SLL is more stable than plain liposome and SLL could coat alcohol dehydrogense and control it release by gas chromatography. The high stability of SLL and possibility for immobilizing biologically active molecules on the crystalline array may offer potential in various different SLL applications.