Abstract
In this paper, we propose a novel method employing photo-induced immune complexes dissociation for surface regeneration. Protons, released by UV irradiation on a photochemical molecule o-Nitrobenzaldehyde (o-NBA), were employed to rapidly elevate solution pH level from 7.0 to 2.7 within 60sec. Applying to the dissociation of antigen/antibody conjugation, the elution efficiency of 85% within 10 minutes can be approached. This method can effectively confine the proton generation region out of reaching most of the target protein molecules in the solution by confining the irradiation of UV light to minimize protein damage, which is easily encountered by traditional acid washing way. Therefore, a reusable immunoassay can be realized.