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利用細胞圖形化磁性薄膜定位細胞
Thesis

利用細胞圖形化磁性薄膜定位細胞

簡伶芳
Masters, 國立清華大學, 奈米工程與微系統研究所
2010

Abstract

細胞圖形化 鐵磁性薄膜 奈米磁性顆粒 cell patterning ferromagnetic thin films nano-magnetic particles
In this thesis, magnetic cell patterning is demonstrated through controlling the micromagnetic states in different micro-structured ferromagnetic thin films. Magnetic nanoparticles entering cells by endocytosis, and the amount of particle inside cells is found dependents on the co-cultured extracellular magnetic nanoparticles concentrations. Including sine waving, zigzag, rhombic, honeycomb, square net, concentric circular and square rings, and single ring shaped magnetic films are designed for testing the magnetic cell patterning. Magnetic films with strong shape anisotropy are magnetized along different in-plane directions to produce different remanent states, which are different types of magnetic domain walls or different magnetic pole densities of films with single domain. It is found that cells can be well patterned by head-to-head or tail-to tail domain walls in waving, zigzag, and ring shaped films, so does by strong poles at rhombic film tips along easy-axis. Besides, domain walls at intersections of films with square net or honeycomb shape also attract cells. Cell patterning also can be achieved on kinds of films if keeping applying an out-of-plane field. In addition, the patterned cells are found elongated and even proliferate along the way with higher magnetic flux density after hours co-culture with films. Results in this thesis can provide important information for future development of tissue engineering and biomedical chips.

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