Abstract
Severe tissue necrosis with a slow wound healing process is a major symptom of a cobra snakebite. Cardiotoxins（CTXs）are major components of cobra venoms that belong to Ly-6 protein family and are implicated in tissue damage. Cobra Cardiotoxin A3 interacts with sulfatide on cell membrane will cause membrane pore foramtion and cell internalization and to be responsible for cytotoxicity in cardiomyocytes. Here we demonstrate a non cytotoxicity cardiotoxin,CTX A5 and its recombinant protein express by E.coli system interact with target, heparin and vintegrin by Surface plasmon resonance analysis and using point mutation technique to mutate the specific protein to observe the difference in binding intensity. We also show the other component of cobra venom, Phosphodiesterase,PDE interact with its potential target,insulin receptor by SPR.