Abstract
Double-waveguide zinc ion sensor combines a light guide plate that has uniformly light-transferring with an optical fiber in parallel. We coat sensing film on the junction between the light guide plate and optical fiber. The sensing film is made from Poly(2-hydroxyethyl methacrylate) which mixed with high selectivity fluorescent probe meso-2,6-Dichlorophenyltripyrrinone. The weight ratio between the two molecular is 0.025 wt%. At first, we use the blue laser diode, which the wavelength is 405 nm and the power is 100mW, as an excitation light source. By using the light guide, the laser can be transferred into the film successfully. After absorbing, the sensing film will be excited and emitting fluorescence. Eventually, the fluorescent light will be coupled into the optical fiber and collected at the other end. The double-waveguide sensor can detect instantly even the concentration of zinc ion with only 10-6M. Moreover, it can sense in Dulbecco’s modified Eagle medium(DMEM) environment. The double-waveguide sensor has a firm structure, so it's not easy to change the shape and to prevent causing abnormal sensing when it faces some force. Besides, the manufacture process of this system is simply and low cost. We use these ideas to achieve the efficacy of real-time zinc ion sensing and some applications in biomedical vivo sensing. Expecting the new kind zinc ion sensor can contribute to the biomedical sensing in vivo or ex vivo.