Abstract
In this study, the goal is to use biosensor as detection for biomolecule binding. The experiment set up includes a circuit board for signaling together with the biosensors. The biosensor is based on a theory of “Shear Horizontal Mode Surface Acoustic Wave” or in short “SH-SAW”. SH-SAW is a high sensitivity biosensor having 36°YX-LiTaO3 as a piezoelectric material fabricated using lithography process. The biosensor can detect the signal by using the frequency shift of surface acoustic wave and result as the amount of biomolecule bindings in graphs. Two biosensors are used at the same time (one is for testing and another as reference) to eliminate the noise that might occur in the environment. There are 2 types of biomolecules used in this study: Bovine serum albumin (BSA) and Epidermal growth factor (EGF). BSA is considered as a non-specific molecule binding so this biomolecule will be used as the double check for the biosensor while EGF is a specific molecule binding will be used as the target. Also, the biosensors are deposited with a thin layer of silicon dioxide (SiO2) and then coated with (3-Aminopropyl) triethoxysilane (APTES) or glutaraldehyde to capture the specific antibody in EGF. Currently, the biosensors can detect a low concentration of 1% BSA solution and 0.1ng/ml of EGF from the frequency respond since it is related to the different concentration of samples.