Abstract
Nitric oxide (NO) and nitrite (NO2-) are important molecules in the human body. Therefore, NO / NO2- sensor in the biomedical research and development has an immense value. This thesis aims to do the research on NO / NO2- waveguide sensing film. The NO probe molecule 1,2-diaminoanthraquinone (DAQ) is dispersed in Poly 2-hydroxyethyl methacrylate (poly HEMA), to form a NO / NO2- hydrogel sensing film which is high selectivity and flexibility, and this film does not be affected by acid. Next, taking the film as the waveguide to increasing the light path and absorbance which enhance the sensing limit and sensitivity. We combine the sensing film with lasers, fiber, and CCD spectrometer as a measurement system for monitoring aqueous NO / NO2- real-time. Nitrite is the main reactant with waveguide sensing.