Abstract
Typically slides, agar-pads are applied to provide cell culture environment under microscope. Even though suitable for short time observation, these systems are limited to more complex experiment. In this work, we provide a microfluidic picoliter bioreactor(PLBR). The device allow continuous medium flow enabling constant environment, but also fast medium changes. We use lithography process to make a mold, the center height of channel is 1um. Make a disposable PLBR by the PDMS soft lithography process. Microfluidic chip were cut, punched, assembled, and integrated with microscope and temperature controller. This is a suitable platform for continuous cultivation of microbial organisms. In addition, our su-8 mold can be reused and only need two days to make the same PLBR. In our bioreactor, we can trap E. coli on single cell level, measure cell growth and fluorescence intensity.