Abstract
The objective of this thesis is to study the stability of the cDNA mcroarray, such as the uniformity of each spot in the microarray, the efficiency of total RNA extraction from tissue or cells and the results of hybridization.Because cDNA microarray technology is new enough, we still have not known the details of it.So we must study the system carefully and find the limits.Then, reasonable analysis of huge gene information is to acquire the most correct gene expression patterns. Knowing spots uniformity during printing, the statistic error of one pin and all variations between pins is the purpose of the microarray uniformity experiment.We can get rid of the systematic error in the final data analysis.The design of this experiment is to spot 40 spots every 20 minutes.Observation of the DNA probe concentration on the microarray have any effects on the system uniformity.The extraction of RNA form tissue or cells is also important in the microarray experiments.RNA used to be labeled fluorescent dye must have enough amount.We use cell line and placenta tissue to estimate the total RNA amount.SKOV3 cell line is one cell type of human ovarian caner.Besides knowing well the extractiontechnology of total RNA, the difference of RNA amout in different samples can be compared.We also compare the results of hybridization bytotal RNA or mRMA labeled fluorescent dye to see the intensity of spots is any different between two RNA types. Because our microarray system is a new one, we can have much more knowledge of the system performance by these basic studies.This is a preliminary study and how to optimize the cDNA microarray technology is still to be worthy of another research and improvement.