Abstract
In recent years, people pay more attention to food safety. Traditional detection technology is expensive, complicated and time-consuming, so that it is impossible to do the real-time and on-site detection for the irregular additives in food samples. This study further investigate and improve the droplet-based piezoelectric polymer deposition. In addition, the deposited piezoelectric polymer deivces are integrated on a biochip for rapid detection of drug residue in foods. First, we changed the wire width of electrode to design and choose different area ratio of P(VDF-TrFE). In the electrodeposition process, we discussed the optimization parameter, including electrodeposition time, volume of droplet, supplying voltage, and the pre-treatment of relative humidity. In the examination of acoustic property, the maximum output power was 86.31 μW (10 kHz). Compare of past study, the signal strengthen was enhanced by 62 % (10 kHz), the output power was enhanced by 37 %, the efficiency was enhanced by 220 %. In the property of acoustic transceiver, the signal coefficient of variation was reduced to 0.38 % from 17.79 % by the pre-tunable treatment. The developed acoustic transceiver was applied to the detection of animal drugs residue in meat samples after the optimization process and pre-tunable treatment. The detection frequency was 10 kHz, and the target drugs were ractopamine、benzylpenicillin、doxycycline. The response, including fluid disturbance and steady tim) was 80 sec, and the SNR of steady signal was up to 29.03 dB. Linear range was 0 ~ 100 ppb, and sensitivity was up to 2.4 mV/ppb and the detection limit was 2.06 ppb (3 dB). Finally, the acoustic transceiver was integrated with MIP films on microfluidic chip, and 20 ppb pork sample was applied to our chip for drugs residue detection. The detection result was up to 23.7±1.15 ppb. The detection time was 4 min, including fluid operation and dynamic signal measurement. In this study, the developed high-sensitivity piezoelectric acoustic transceiver presented the advantages of low cost, simple steps and high integration. Besides the drugs residue detection, it also was applied as others biosensor in the future.