Abstract
Recently, the research of single molecule techniques has developed rapidly and now provides an innovative stage for bio-medical researches. Single molecule approach may provide a potential way and novel solution to developing DNA sequencing techniques that may probably be a powerful tool for genome research in the present day. However, at the present stage there still exist many obstacles that deserve to overcome. In this study, based on the capabilities of the DNA manipulation, together with the optical tweezers system, established in our core Lab, we will propose a new sequencing method with the help of exonuclease and laser excitation such that one can clip the single nuclide of a DNA and put them on the CD-ROM for ultra-fast and precision sequencing. Hope that this approach via macro and preliminary observation as a first step may be formed the basis of the following single molecule detection studies. This thesis contains three major sections, the brief introduction of DNA sequencing and the list of the difficulties of the current single molecule technique, the fundamental idea and the preliminary strategy of DNA CD-ROM sequencing, and the study of “Laser Assisted Exonuclease Activities”, respectively. In this thesis, the key point is to find out the important related parameters in an effort to control the activity of exonuclease included the assist of laser. Finally, the results of the experiences concerning an “Optimization of DNA Hybridization Protocol for Microarray System” are clearly described in the appendix 4.