Abstract
In this research we propose the utilization of iron oxide nanoparticles for the enhancement of protein binding efficiency to SAMs functionalized surface. Very uniform iron oxide nanoparticles with 5 and 10 nm particle sizes have been successfully fabricated by using micelle method in solvent phase, and the surfaces were modified by PEG-COOH functional group for dispersion in water and conjugation with protein. Anti-rabbit IgG-cy3 was successfully conjugated on the nanoparticle surfaces after the activation of PEG-COOH group. Binding efficiency between iron oxide-protein conjugation and self-assembly molecules (SAM) by external magnetic field with different AC frequencies was tested and demonstrated a 374% enhancement.