Abstract
In this study, we divided as mono-culture and BBB model system to explore the toxicity effect of TiO2 (3-5nm ST-01, 30-50nm ST-21) and Ag NPs (<10nm). In vitro BBB model by co-culture endothelial cells (bEnd.3) and astrocytes (ALT) were established to estimate whether BBB dysfunction. Accordingly, four works will be examined after exposing nanoparticles 24 hours, all of which are cell viability, uptake potential, intracellular reactive oxygen species (ROS), and cytokines (MCP-1). In addition, the integrity of BBB was estimated by measuring the tranendothelial electrical resistance (TEER), calculating the permeability and observing the tight junction protein expression. On the other hand, we also compare the condition with or without lipopolysaccharide (LPS), which is assumed as the inflammatory situation. Consequently, we found that the toxicity mechanism of Ag-NPs and TiO2 NPs in the cells were trend to follow the way by producing ROS to gradually induce cell death. BBB were influenced by this two kind of nanoparticles to decrease TEER value, disrupt tight junction proteins and increase permeability of nanoparticles. The reason of this were associated with ROS generation and cytokines secretion. Furthermore, we found that Ag-NPs were more noxious than TiO2 NPs according to the lower lethal dosage (2ppm) and higher permeability in the BBB model. On the other hand, LPS treatment would stimulate MCP-1 release to make severer effect on BBB model although it did not have significant influence with co-culture nanoparticles.