Abstract
We apply a current simple method, quick diversity-oriented amide-forming reaction, for preparation in solution of a library of compounds. These compounds are synthesized through a coupling reaction between a 5’-amino uridine and 24 kinds of carboxylic acids, followd by a direct assay of cytotoxicity without product purification. We hope that this innovative developing approach can screen potential drugs for cancer therapy. The human non-small cell lung tumor cell A549 is our choice as a targer. In order to eliminate the toxicity of solvent DMF (dimethylformamide) to A549, one strategy adopted is to make DMF become a quaternary salt by adding HCl. Althoughg this treatment overcomes the toxicity of DMF, the effect is the abnormal physiological condition where A549 growing is between pH=4 and pH=5. Therefore, MTT assay isn’t carried out under this condition, and we analyze the reasons by full spectrum. The cell viability is recorded only with microscopic observation. More fortunately, the reagents used seem not to be toxic to A549. Finally, among the 24 kinds of solutions as drugs added into 96-well plate containing A549, we don’t find out anyone possessing apparent cytotoxicity. After we roughly completed these tests, there are still many problems and difficulties have to be overcomed and make the whole experimental concept more optimized.