Abstract
DNA replication and partition played an important role in cell division. To ensure the amounts of genetic material in daughter cells are equal, Par(partition) system makes the replicated DNA moves to daughter cells precisely. Par system contains Spo0J, Soj and centromere-like parS DNA sequence. HpSpo0J and HpSoj are the two proteins from H.pylori for Par system. Soj is an ATPase and binding to nonspecific DNA sequence. Spo0J is a DNA binding protein for parS DNA binding specifically and it can stimulate the ATP hydrolysis of Soj. Using electrophoretic mobility shift assay to detect the parS DNA (15 and 17bp) binding to HpSpo0J. Also, a 55bp parS DNA was used to study the DNA binding to HpSpo0J and HpSoj, respectively by EMSA. The ATPase activity of HpSoj was performed by the molybdenum-blue assay and the ATPase activity of 0.96 ± 0.3 mole Pi/ mole of Soj．hr was determined. HpSpo0J or parS-55bp DNA will stimulate the ATPase activity. The additional of HpSpo0J and parS-55bp DNA mixed the ATPase activity is increased 45 folds the ATPase activity of HpSoj. We speculate that HpSpo0J, HpSoj and parS-55bp may work corporately. Unfortunately, the crystallization of the protein-nucleotide complex, the Spo0J-Soj-parS DNA complex was fail. The fail of crystallization might because the instability of HpSpo0J and the long length of parS-55bp.