Abstract
Lactic acid bacterial is a kind of probiotic bacteria and wide exists in nature. In this research, we constructed a vector, pAC, which can exist between Lactic acid bacterial and E.coli. The pAC vector was constructed by using the pAMβ1 replicon and the pUC origin. In my result, pAC can exist in Lactococcus lactis MG1363 but not in Lactobacillus rhamnosus TCELL-1. In addition, we also use the pAC vector to study methods for gene transfer such as electroporation、conjugation and gene gun on the application of lactic acid bacteria. The result shows that electroporation and conjugation methods were successive in MG1363 but not in TCELL-1. And with gene gun method, it may be restricted by the Au or W particle size and not suitable for the species. The reason that TCELL-1 is difficult to be transformed is not known. It will require further study.