Abstract
The biomedical science will bring a revolutionary changes in the 21st century. Besides the bioinformatics techniques, gene techniques, chip microfabrication and robotics techniques, which have already been practic applied in the medical field, the tissue engineering shows a promising future. Using the BioMEM microfabrication techniques for implantable biohybrid organs becomes an exacting technology. However, due to the lack of blood circulation system, these artificial tissues or organs cultured in vitro, suffering from the low nutrient and oxygen exchange efficiency, head to cell apoptosis quickly. In BioMEMES microfabrication, distortion always happened owing to the microfluidic channel unable to appropriate adopt a good permeability material to simulate the actual blood vessel. Due to the above problems, we use a material with good permeability for the microchannel. We have also combined gelatin coating, cell arrangement and cell seeding techniques to uniformly coat on the microchannel’s inner wall. This simulates the blood vessel to avoid thrombus effect. We use PDMS（Polydimethylsiloxane）as the substrate which has a very good permeability and biocompatibility characters to fabricate microchannel. We conduct numerical simulation of the microchannel by using CFDRC to find the optimum velocity and time distribution. In static cells culture experiment, we found that endothelial cells can be seeded within the microchannel successfully; however, the seeding coverage was not high enough. Therefore, we try to improve it by dynamic cell culture experiment. In dynamic cells culture experiment, we changed the culture solution by syringe pump every eight hours, and it has a better seeding coverage of about 60%-70%. Finally, we injected blood into the microfabricated cell seeded artificial lung device and checked the blood oxygenation level-dependency （BLOD）to measure the difference between the inlet and outlet of the microchannel. The preliminary result shows that the device can serve an artificial lung’s basic function of gas exchanged. The average BOLD value （SpO2）rises to 94.5 from 90.2..