Abstract
With the rapid growth of nanotechnology development, numerous products contained nanoparticles are widely used in our daily life. However, the nanoparticle can accumulate in our bodies via exposure of nanoparticle in environment . The accumulation of nanoparticles can cause cell damage, degenerative diseases, and even cancer progression. Thus, there is an increasing concern about the nanoparticle utilization recently, and an emerging need for nanotoxicity detection. In this study, we aimed to develop a cell-based nanotoxicity biosensor with high detection sensitivity. Micro contact printing (mCP) was applied to pattern liver cells into specific shape, and the nanotoxicity of nanoparticle was quantified with fluorescent ROS indicator using fluorescent microscopy. Our results demonstrated, comparing to control group, the patterned cells have higher sensitivity to nanoparticles. Under the pressure of TiO2, our designed cell can detect (0.625 mg/L) TiO2 presented in the medium. We further investigated the underlying mechanism of sensitivity enhancement. With FRAP microscopy, decreasing actin turnover of pattern cells was observed, and the slower actin dynamics is associated with ROS expression. In addition, considering the spatial organization of mitochondria within patterned cells, the mitochondrial RIRR model may contribute to the ROS generation. In summary, we demostrated that the micron-scale patterned cells fabricated by mCP, can be ultilized as a more effective nano-toxicity biosensor, and observed the rearrangement of cytoskeleton and actin dynamic in cells are highly associated with cell physiological functions.