Abstract
The protein chip is a micro-chip with its surface modified to detect various disease causing proteins simultaneously in order to help find a cure for them. This thesis is going to introduce a back-filling protein chip without any external driving force. Bio-MEMS technique makes a batch of cheap chips, and they are disposable form samples cross contamination. The flow channels are made by SU-8；PDMS polymer was used for the array at the bottom. First of all, the protein samples were lead into the upper reservoirs by a pipette. Next the samples flowed through the micro-channels by the capillary force and arrived the array at the bottom. Finally different protein arrays would come out like the term of printing stamps. This research improves the quality of arrays and summarizes the problems of the faults of the arrays. The used protein solutions are Cy3-tagged anti-rabbit IgG and Cy5-tagged anti-mouse IgG. Now this chip can successfully demonstrate three types of arrays without cross-talk, including single-protein array, multi-protein arrays and cancer-marker arrays. Each spot is formed by a single droplet about 1nl at each position. These protein arrays is characterized with a deviation ±3% in size and 20% in fluorescent intensity. The cancer antigens were made by chip stamping and using ELISA (Enzyme Linked- Immuno -Sorbent Assay) method the cancer levels down to 1ng/μl were detected.